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. Author manuscript; available in PMC: 2011 Nov 1.
Published in final edited form as: J Allergy Clin Immunol. 2010 Nov;126(5):1049–105910. doi: 10.1016/j.jaci.2010.07.023

Figure 7. Direct Effects of TSLP on Purified T Cells Induced in a “Pro-Allergic” Milieu.

Figure 7

(A) Purified T cells that had been pre-activated with TSLP+allergen were not stimulated or re-stimulated with TSLP or IL-2 for 0 to 30 minutes and analyzed for phosphorylated STAT5. Values represent the percentage of cells positive for phospho-STAT5 following re-stimulation (values for non-stimulated cells are shown in parentheses for each corresponding time point). Data is representative of 3 experiments. (B) Culture supernatants from purified T cells that had been pre-activated sequentially with TSLP+allergen followed by anti-CD3+anti-CD28 were assayed for cytokines following re-stimulation with TSLP or IL-2 for up to 6 hours. NS, not stimulated.