FIG. 3.

Southern blot analyses of VVCL1. (A) The HCMV genome contains unique long (U_L) and short (U_S) segments flanked by inverted repeats (ab, b′a′c′, ca). The locations of the HCMV cosmid inserts and of the EcoRI/ApoI fragment present in VVCL1 are shown. (B) DNAs from HCMV and from HCMV cosmids were digested with EcoRI and XhoI, separated electrophoretically, stained with ethidium bromide (top), and then hybridized with ³²P-labeled VVCL1 DNA. Autoradiography (bottom) identified specific bands in Tn46 and Tn15 (*). (C) DNA from HCMV or from the indicated VV was digested with HindIII and then viewed by ethidium bromide staining (top) and hybridized with TRS1 (middle) and J1S (bottom) probes. VCL1 and HCMV, but none of the other VVs, contained TRS1 and IRS1 sequences. Molecular size markers (in kilobases) are indicated.