FIG. 7.

Analysis of p18-specific CD8⁺-T-cell central memory and effector memory subsets elicited by DNA vaccination with or without delayed administration of plasmid IL-12. Groups of BALB/c mice were immunized as described in the legend to Fig. 6. Peripheral blood was obtained from individual mice at the indicated times, and expression of CD62L on gated H-2D^d/p18 tetramer-positive CD8⁺ T cells was measured by flow cytometry. Data are presented as the percentage of CD8⁺ T cells that were either tetramer positive and CD62L⁺ or tetramer positive and CD62L⁻ and are the means of eight mice per group ± SEM. d., day.