TABLE 2.

Phenotypic analysis of p18-specific CD8⁺ T cells isolated from various lymphoid and nonlymphoid tissues following DNA vaccination with or without delayed administration of plasmid IL-12

Tissuea	% of CD8+ T-cell populationb
	Tetramer+ CD62L+ central memory		Tetramer+ CD62L− effector memory
	Sham plasmid	Plasmid IL-12	Sham plasmid	Plasmid IL-12
Lymphoid
Peripheral LN	0.05 ± 0.01	0.05 ± 0.01	0.02 ± 0.01	0.02 ± 0.01
Mesenteric LN	0.05 ± 0.01	0.06 ± 0.01	0.02 ± 0.01	0.02 ± 0.01
Spleen	0.15 ± 0.05	0.21 ± 0.03	0.53 ± 0.12	1.50 ± 0.22
Nonlymphoid
Blood	0.08 ± 0.01	0.13 ± 0.04	0.71 ± 0.10	3.75 ± 0.78
Bone marrow	0.23 ± 0.07	0.31 ± 0.11	1.21 ± 0.32	2.99 ± 0.56
Peritoneum	1.12 ± 0.16	1.07 ± 0.07	4.71 ± 0.89	6.06 ± 0.67
Lung	0.17 ± 0.02	0.30 ± 0.04	2.43 ± 0.48	12.82 ± 1.46
Liver	0.52 ± 0.13	0.64 ± 0.04	1.84 ± 0.31	6.20 ± 1.04

^aMice were immunized with 50 μg of PMV-gp120 plus 200 μg of sham PMV plasmid or 200 μg of PMV-IL-12 on day 10 following vaccine administration. The indicated lymphoid and nonlymphoid tissues were harvested from animals on day 34 postimmunization, and lymphocytes were isolated as described in Materials and Methods.

^bExpression of CD62L on gated H-2D^d/p18 tetramer-positive CD8⁺ T cells from the indicated tissues was analyzed by flow cytometry. Data are presented as the percentage of CD8⁺ T cells that were tetramer positive CD62L+ or tetramer positive CD62L⁻ and represent the means of four mice per group ± SEM.