TABLE 3.
p18-specific CD8+ T cells isolated from various lymphoid and nonlymphoid tissues following plasmid gp120 boost immunization
| Tissuea | % Tetramer+ CD8+ T cellsb
|
|
|---|---|---|
| Sham plasmid | Plasmid IL-12 | |
| Lymphoid | ||
| Peripheral LN | 0.46 ± 0.02 | 0.34 ± 0.10 |
| Mesenteric LN | 0.66 ± 0.04 | 0.63 ± 0.19 |
| Spleen | 8.70 ± 0.24 | 10.70 ± 1.06 |
| Nonlymphoid | ||
| Blood | 12.94 ± 1.38 | 15.50 ± 0.08 |
| Bone marrow | 8.76 ± 0.05 | 14.02 ± 2.38 |
| Peritoneum | 29.54 ± 3.29 | 22.53 ± 2.70 |
| Lung | 34.80 ± 3.07 | 53.80 ± 3.56 |
| Liver | 29.70 ± 1.71 | 35.12 ± 1.91 |
Mice were immunized with 50 μg of PMV-gp120 plus 200 μg of sham PMV plasmid or 200 μg of PMV-IL-12 on day 10 following vaccine administration. On day 145 postimmunization, mice were boosted with 50 μg of PMV-gp120. The indicated lymphoid and nonlymphoid tissues were harvested from animals on day 10 following boost immunization, and lymphocytes were isolated as described in Materials and Methods.
Antigen-specific CD8+ T cells were detected by using an H-2Dd/p18 tetramer. Data are presented as the percentage of gated CD8+ T cells that bound tetramer, as measured by flow cytometry, and are the means of four mice per group ± SEM.