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. Author manuscript; available in PMC: 2012 Jan 17.
Published in final edited form as: Vaccine. 2010 Nov 27;29(4):681–689. doi: 10.1016/j.vaccine.2010.11.030

Fig. 4. Intestinal immunity to ricin following parenteral immunization with RiVax.

Fig. 4

Groups of Balb/c mice (n= 6–9/group) were immunized by the s.c. route three times with RiVax (10 µg/immunization) or PBS. Serum was collected 7–10 days following each immunization. (A) Anti-ricin serum IgG antibody titers were determined by ELISA, as described in Materials and Methods. Shaded circles reflect immunized mice, whereas open triangles reflect sham-immunized controls. Each symbol represents a single mouse. The solid, horizontal bars reflect the average of the groups. (B) Groups of RiVax or sham immunized mice were challenged with ricin (5 mg/kg) by gavage. A third group of mice not immunized or challenged served as negative controls for this experiments. MCP-1 levels in intestinal tissues from all three groups of mice were determined 24 hr following intragastric ricin challenge, as described in Materials and Methods. The asterisks indicate that MCP-1 levels in control, toxin-challenged mice were statistically different from RiVax-immunized mice (p<0.05), as determined by the Student’s t test.