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Effect of RtsA on production of DsbA: Western blot analyses of culture supernatants (top panel) or whole-cell extracts (bottom panel) with anti-DsbA and anti-β-lactamase antibodies. Except for the ATCC 14028 control, the strains are ΔrtsAB and contain the plasmids and mutations indicated above the lanes. Equivalent amounts of sample were separated by SDS-12.5% PAGE. The strains used were plasmid-containing derivatives of JS250, JS332, JS333, and JS334.
