FIG. 4.

Effect of a dsbA mutation on translocation of SPI1 (A) and SPI2 (B) TTS effectors. The strains contained the protein fusions to the CyaA catalytic domain indicated below the graphs. For panel A the strains were either wild type or contained a deletion of hilC-D (which removed hilC-prgHIJK-hilD), dsbA, or cpxR, and they were grown under SPI1-inducing conditions. For panel B the strains were either wild type or contained a deletion of ssaT (a SPI2 TTSS structural gene), dsbA, or cpxR and were grown under SPI2-inducing conditions. The cAMP levels were determined as described in Materials and Methods. The strains used were JS355 through JS363. WT, wild type.