TABLE 1.
Strains and plasmids
| Strain or plasmid | Genotype or relevant characteristicsa | Deletion or cloned end pointsb | Source or referencec |
|---|---|---|---|
| Strains | |||
| ATCC 14028 | Wild type serovar Typhimurium | ATCCd | |
| JS248 | ΔrtsA3 | 4561755-4560884 | 26 |
| JS250 | ΔrtsAB7 | 4561769-4560602 | 26 |
| JS251 | ΔhilA112::Cm | 3019885-3021480 | 26 |
| JS252 | ΔhilC113::Cm | 3012135-3012976 | 26 |
| JS253 | ΔhilD114::Cm | 3017865-3018730 | 26 |
| JS254 | ΔinvF100::Cm | 3043931-3043290 | 26 |
| JS256 | ΔhilC-D2915::Cm | 3012135-3018730 | 26 |
| JS275 | ΔrtsAB7 φ(hilA-lac+)112 | 26 | |
| JS276 | ΔrtsAB7 φ(invF-lac+)100 | 26 | |
| JS279 | φ(hilA-lac+)112 | 26 | |
| JS282 | φ(invF-lac+)100 | 26 | |
| JS285 | sopA100::MudJ | 26 | |
| JS289 | sopB101::MudJ | 26 | |
| JS302 | ΔrtsAB7 ΔhilC-D2915::Cm φ(hilA-lac+)112 | 26 | |
| JS304 | ΔrtsAB7 ΔhilC-D2915::Cm φ(invF-lac+)100 | 26 | |
| JS306 | ΔrtsAB7 sopA100::MudJ | 26 | |
| JS308 | ΔrtsAB7 ΔinvF100::Cm sopA100::MudJ | 26 | |
| JS309 | ΔrtsAB7 sopB101::MudJ | 26 | |
| JS311 | ΔrtsAB7 ΔinvF100::Cm sopB101::MudJ | 26 | |
| JS318 | ΔrtsAB7 φ(slrP-lac+)100 | 26 | |
| JS319 | ΔrtsAB7 ΔhilC-D2915::Cm φ(slrP-lac+)100 | 26 | |
| JS320 | ΔrtsAB7 ΔinvF100::Cm φ(slrP-lac+)100 | 26 | |
| JS322 | ΔrtsA5 ΔhilC-D2915::Cm φ(hilA-lac+)112 | 26 | |
| JS326 | ΔdsbA100::Cm | 4204198-4204820 | |
| JS327 | ΔcpxR100::Cm | 4270445-4269745 | |
| JS328 | ΔbaeR100::Cm | 2224566-2225230 | |
| JS329 | ΔpspF100::Cm | 1782615-1783537 | |
| JS330 | Δ(sitA-pphB) | 3006017-3048180 | |
| JS331 | ΔdsbB101::Cm | 1908529-1909020 | |
| JS332 | ΔrtsAB7 ΔhilC-D2915::Cm | ||
| JS333 | ΔrtsAB7 ΔcpxR100::Cm | ||
| JS334 | ΔrtsAB7 ΔdsbA100::Cm | ||
| JS335 | ΔrtsA5 ΔhilC-D2915::Cm φ(dsbA-lac+)100 | ||
| JS336 | ΔrtsAB7 φ(dsbA-lac+)100 | ||
| JS337 | ΔrtsAB7 ΔcpxR100::Cm φ(dsbA-lac+)100 | ||
| JS338 | ΔrtsAB7 ΔhilA112::Cm φ(dsbA-lac+)100 | ||
| JS339 | ΔrtsAB7 ΔpspF100::Cm φ(dsbA-lac+)100 | ||
| JS340 | ΔrtsAB7 ΔbaeR100::Cm φ(dsbA-lac+)100 | ||
| JS341 | ΔrtsAB7 ΔsitA-pphB::Cm φ(dsbA-lac+)100 | ||
| JS342 | ΔrtsAB7 attλ::pCE83 | ||
| JS343 | ΔrtsAB7 attλ::pCE84 | ||
| JS344 | ΔrtsAB7 attλ::pCE85 | ||
| JS345 | ΔrtsAB7 attλ::pCE86 | ||
| JS346 | ΔrtsAB7 attλ::pCE87 | ||
| JS347 | ΔrtsAB7 attλ::pCE89 | ||
| JS348 | ΔrtsAB7 attλ::pCE90 | ||
| JS349 | ΔrtsAB7 attλ::pCE91 | ||
| JS350 | ΔrtsAB7 attλ::pCE92 | ||
| JS351 | ΔrtsAB7 attλ::pAH125 | ||
| JS352 | MC4100 ara+ | ||
| JS353 | JS352 attλ::λRS88(dsbA′-lacZ+) | ||
| JS354 | JS352 attλ::pCE83 | ||
| JS355 | pCE39 | ||
| JS356 | slrP+::pTH807 | ||
| JS357 | slrP+::pTH807 ΔhilC-D2915::Cm | ||
| JS358 | slrP+::pTH807 ΔdsbA100::Cm | ||
| JS359 | slrP+::pTH807 ΔcpxR100::Cm | ||
| JS360 | pSG161 | ||
| JS361 | pSG161 ΔssaT100::Kn | ||
| JS362 | pSG161 ΔdsbA100::Cm | ||
| JS363 | pSG161 ΔcpxR100::Cm | ||
| JS364 | pZP188 ara623::Tn10dTc | ||
| JS365 | pZP188 ΔdsbA100::Cm ara623::Tn10dTc | ||
| JS366 | pZP188 ΔhilC-D2915::Cm ara623::Tn10dTc | ||
| JS367 | pZP188 ΔcpxR::Cm ara623::Tn10dTc | /PICK> | |
| JS368 | pZP212 ara623::Tn10dTc | ||
| JS369 | pZP212 ΔdsbA100::Cm ara623::Tn10dTc | ||
| JS370 | pZP212 ΔhilC-D2915::Cm ara623::Tn10dTc | ||
| JS371 | pZP212 ΔcpxR100::Cm ara623::Tn10dTc | ||
| JS372 | sopA100::MudJ ΔdsbA100::Cm | ||
| JS373 | sopA100::MudJ ΔinvF100::Cm | ||
| JS374 | sopB101::MudJ ΔdsbA100::Cm | ||
| JS375 | sopB101::MudJ ΔinvF100::Cm | ||
| JS376 | φ(slrP-lac+) | ||
| JS377 | φ(slrP-lac+) ΔdsbA100::Cm | ||
| JS378 | φ(slrP-lac+) ΔinvF100::Cm | ||
| JS379 | φ(hilA-lac+)112 ΔdsbA100::Cm | ||
| JS380 | φ(invF-lac+)100 ΔdsbA100::Cm | ||
| JS381 | ΔrtsAB7 ΔdsbA100::Cm sopA100::MudJ | ||
| JS382 | ΔrtsAB7 ΔhilC-D2915::Cm sopA100::MudJ | ||
| JS383 | ΔrtsAB7 ΔdsbA100::Cm sopB101::MudJ | ||
| JS384 | ΔrtsAB7 ΔhilC-D2915::Cm sopB101::MudJ | ||
| JS385 | ΔrtsAB7 ΔdsbA100::Cm φ(slrP-lac+)100 | ||
| JS386 | ΔrtsAB7 ΔdsbA100::Cm φ(hilA-lac+)112 | ||
| JS387 | ΔrtsAB7 ΔdsbA100::Cm φ(invF-lac+)100 | ||
| MC4100 | F−araD139 Δ(argF-lac)U169 rpsL150 relA1 | 67 | |
| flhD5301 deoC1 ptsF25 rbsR | |||
| PAD281 | MC4100 λRS88(dsbA′-lacZ+) | 16 | |
| Plasmids | |||
| pKD46 | bla PBADgam bet exo pSC101 onTS | 22 | |
| pCP20 | bla cat cI857 λPRflp pSC101 on TS | 11 | |
| pKD3 | bla FRT cat FRT PS1 PS2 oriR6K | 22 | |
| pCE36 | ahp FRT lacZY+ this oriR6K | 25 | |
| pCE37 | ahp FRT lacZY+ this oriR6K | 25 | |
| pBAD30 | bla araC PBAD pACYC184 ori | 30 | |
| pRtsA | bla PBAD λAttB1 rtsA+ λAttB2 pACYC184 ori | 4561766-4560885 | 26 |
| pRtsB | bla PBAD λAttB1 rtsB+ λAttB2 pACYC184 ori | 4560890-4560595 | 26 |
| pRtsAB | bla PBAD λAttB1 rtsA+B+ λAttB2 pACYC184 ori | 4561766-4560595 | 26 |
| pZP188 | bla PBADsopA-M45 pACYC184 ori | D. Zhou | |
| pZP212 | bla PBADsopB-M45 pACYC184 ori | D. Zhou | |
| pCE39 | bla PLAClacZ′-cyaA pACYC184 ori | ||
| pSG161 | bla PLACsspH2′-cyaA pACYC184 ori | ||
| pTH807 | aph slrP′-cyaA oriR6K | ||
| pAH125 | lacZ tL3 λattP oriR6K Kan tmgB | 32 | |
| pLS118 | bla PBADhilD-myc/his pMB1 ori | 65 | |
| pLS119 | bla PBADhilC-myc/his pMB1 ori | 65 | |
| pCE83 | lacZ tL3 λattP oriR6K Kan tmgB | 4202771-4204242 | |
| pCE84 | lacZ tL3 λattP oriR6K Kan tmgB | 4202771-4204125 | |
| pCE85 | lacZ tL3 λattP oriR6K Kan tmgB | 4202771-4204067 | |
| pCE86 | lacZ tL3 λattP oriR6K Kan tmgB | 4202771-4203811 | |
| pCE87 | lacZ tL3 λattP oriR6K Kan tmgB | 4202771-4203291 | |
| pCE89 | lacZ tL3 λattP oriR6K Kan tmgB | 4203102-4204067 | |
| pCE90 | lacZ tL3 λattP oriR6K Kan tmgB | 4203342-4204067 | |
| pCE91 | lacZ tL3 λattP oriR6K Kan tmgB | 4203675-4204067 | |
| pCE92 | lacZ tL3 λattP oriR6K Kan tmgB | 4203748-4204067 |
a
Unless otherwise noted, all strains are isogenic derivatives of ATCC 14028.
b
The numbers indicate the base pairs that are deleted or cloned (inclusive) as defined in the serovar typhimurium LT2 genome sequence in the National Center for Biotechnology Information database. The end points are deletion end points for strains and cloned endpoints for plasmids.
c
This study, unless otherwise noted.
d
ATCC, American Type Culture Collection.