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. Author manuscript; available in PMC: 2011 Feb 7.
Published in final edited form as: Br J Haematol. 2009 Sep 29;148(1):132–143. doi: 10.1111/j.1365-2141.2009.07925.x

Fig 1.

Fig 1

MS-1020 inhibits STAT92E transcriptional activity in cultured Drosophila cells. (A) Crude extracts (CE) of Phragmites communis Trin. inhibit Upd (cytokine)-induced STAT92E reporter activity in a dose-dependent manner. Cultured Drosophila S2-NP-STAT92E cells expressing a STAT92E reporter gene were co-cultured for 24 hours with Upd-producing cells in the presence of Phragmites communis Trin. extracts at different concentrations. The STAT92E reporter activity was normalized as the ratio of firefly luciferase to Renilla. The reporter activity without Upd stimulation was set to 1. Results are shown as the mean of three independent experiments (± SD indicated by error bar). (B) The chemical structures of Nb-(p-coumaroyl)serotonin, Nb-(p-feruloyl)serotonin, and MS-1020. Nb-(p-coumaroyl)serotonin and Nb-(p-feruloyl)serotonin were isolated as active compounds responsible for JAK/STAT signaling inhibition, from the extracts of Phragmites communis Trin. A derivative, MS-1020 was chemically synthesized (see Materials and Methods). (C) Dose effect of MS-1020 on Upd-induced STAT92E reporter activity. S2-NP-STAT92E cells were co-cultured for 24 hours with Upd-producing cells in the presence of either vehicle (DMSO) alone or MS-1020 at various concentrations up to 50 μmol/Lol/L. Results are shown as the mean of three independent experiments (± SD indicated by error bar).