Fig. 5. Transcripts encoding ErbB3 can be detected in rat fetal bowel and immunoisolated ENCDC.

RNA was extracted from the whole bowel at E12, E17, and P1 and from ENCDC immunoselected from the gut at E15 with antibodies to p75^NTR. RT-PCR was used to analyze expression of ErbB3. A. Transcripts encoding ErbB3 were detected in the whole gut at each age examined. Note that no products of amplification appeared in controls when RT was omitted (RT−). B. Transcripts encoding ErbB3 were found primarily in immunoisolated ENCDC (CD), although some can also be found in negatively immunoselected non-crest-derived cells (nCD). Despite the negative selection, the nCD preparation contained some residual transcripts encoding p75^NTR and thus was slightly contaminated with ENCDC.