Table 2.
Antibodies used to identify enteric glia and neurons
| Antigen/Species | Host | Dilution | Source | References |
|---|---|---|---|---|
| p75NTRextracellular domain/mouse | Rabbit polyclonal | 1:1000* | Moses Chao, Skirball Institute New York University, NY | Huber and Chao, 1995; Chalazonitis et al., 2008 |
| 1:2000 | ||||
| p75NTR/rat PC12 membranes | Mouse monoclonal clone MC192 | 20 µg/ml* | Robert A. Rush, Flinders University, Adelaide, Australia | Chandler et al., 1984; Chalazonitis et al., 2001 |
| BFABP recombinant protein/mouse | Rabbit polyclonal | 1:170 | Thomas Müller, Max Delbrück Center for Molecular Med., Berlin | Kurtz et al., 1994; Young et al., 2003 |
| S100 (A and B) cow brain | Rabbit polyclonal | 1:1000 | DAKO Glostrup Denmark Cat# Z 0311 | Young et al., 2003 |
| S100 β purified recombinant protein/Human | Rabbit polyclonal | 1:2000 (whole mount) | Cat# A 5110 | |
| 1:400 (cultures) | ||||
| GFAP purified/bovine | Rabbit polyclonal | 1:1000 (cultures) | Chemicon/Millipore Temecula, CA Cat# AB5804 | Jungling et al., 2003 |
| GFAP purified glial filament/human | Mouse monoclonal clone G-A-5 | 1:150 (cultures) | Chemicon/Millipore Cat#MAB3402 | Yang et al., 2005 |
| GFAP purified | Chicken IgY Polyclonal | 1:1000 (sections) | Neuromics, Edina MN, Cat# CH22102 | Liu et al. 2009 |
| PH-3 peptide (aa7–20) of P Histone-3/Human | Rabbit polyclonal | 1:200 (cultures) | Upstate, cell signal. solutions/Millipore Lake Placid, NY Cat#06-570 | Bondurand et al., 2003 |
| ErbB3 p160 (17aa peptide in last 50 aa of C-terminal domain)/Human | Mouse monoclonal Clone G4 | 1:50 (cultures) | Santa Cruz Biotechnology, Inc. Santa Cruz, CA Cat# SC-7390 | Ritch et al., 2003 |
| ErbB3 (19aa peptide of N-terminal domain/Human | Rabbit polyclonal | 1:50 (sections) | Abnova, Taiwan Corp. Taipei, ROC | |
| Sox10, epitope first 65 aa/mouse or rat | Mouse monoclonal Clone 20B7 | 1:10 | David J. Anderson, California Institute of Technology HHMI, CA | Lo et al., 2002 |
| pSmad1,5,8 peptide residues around P Ser 463/465 and P Ser426/428/Human | Rabbit polyclonal | 1:100 | Cell Signaling Technology, Inc. Beverly, MA Cat#9511 | Ahn et al., 2001 |
| HuC/HuD, RNA binding protein of the Elav family/Human | Mouse monoclonal biotinylated clone 16A11 | 1:50 | Molecular Probes, Eugene, OR Cat#A21272 | Wakamatsu et al., 2001 |
| PGP 9.5 purified from brain/Human | Rabbit polyclonal | 1:800 | Biogenesis Ltd, Poole,UK Cat#7863-0504 | Wilkinson et al., 1989 |
*
Dilution used for immunoselection.