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Velocity of RecA-mediated 434 repressor autoproteolysis depends on the concentration of the repressor-DNA complex. Different concentrations of OR1 were added to 50 nM (▪), 250 nM (▴), and 2 μM (▾) (fixed concentrations) 434 repressor in the presence of 250 nM RecA*. The observed cleavage velocities are plotted as a function of repressor-OR1 complex concentration.
