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VEGF-A expression by HemSCs is regulated by NF-κB. Panel A, HemSCs treated with Velcade (5nM) for three days were assayed by RT-qPCR for VEGF-A transcripts. Panel B, an ELISA for VEGF-A protein was performed on conditioned media from HemSCs treated with Velcade. Panel C, HemSCs treated with quinazoline (QNZ), a specific inhibitor of NF-κB activity, were assayed for VEGF-A mRNA by RT-qPCR. Panel D, HemSCs treated with QNZ activity, were assayed for VEGF-A protein by ELISA.
