Figure 1. L1 expression and retrotransposition in EC cells.
(a) ECs express endogenous L1 ORF1p. The ribosomal S6 protein is a loading control. MW=molecular weight standards. (b) Results of the retrotransposition assay in HeLa and EC cells. G418-resistant foci that expressed the retrotransposed NEO reporter gene were stained for visualization. (c) PCR assay for intron removal (retrotransposition) in both HeLa and PA-1 cells. LRE3=a retrotransposition-competent L1. JM111=a retrotransposition-defective L1. MW= 1 kb molecular weight ladder.