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. Author manuscript; available in PMC: 2011 Feb 7.
Published in final edited form as: Nature. 2010 Aug 5;466(7307):769–773. doi: 10.1038/nature09209

Figure 2. Engineered L1 retrotransposition events are efficiently silenced in EC cells.

Figure 2

(a) A cartoon of an L1 and the experimental rationale (top). Cells were transfected with an RC-L1 (kpLRE3/mEGFPI, top two panels; cpLRE3/mEGFPI, bottom panel) and were untreated (left panel) or treated with TSA (right panel). The percentage of EGFP-positive cells and standard deviation (n=3) is indicated. Hoechst staining (blue) highlights the nuclei of cells. P=experiments where puromycin was used to select for the episomal L1 expression plasmid. (b) Retroviral insertions are not efficiently silenced in PA-1 cells (see methods). The graphs indicate the percentage of EGFP-positive cells and the standard deviation (n=3).