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. Author manuscript; available in PMC: 2011 Aug 1.
Published in final edited form as: Proteomics. 2010 Aug;10(15):2746–2757. doi: 10.1002/pmic.200900419

Figure 5. Mapping of the FBXO25 deletion constructs for activation in the yeast two-hybrid screen.

Figure 5

(A) Schematic drawing of FBXO25 mutant lacking the F-box domain (ΔF) and C-terminally truncated versions of FBXO25 fused to LexA DNA binding domain in plasmid pBTM116. (B) Results of the test for reporters gene activation by different FBXO25 constructs. The L40 yeast strain was cotransfected with the indicated pBTM116-FBXO25 and pACT2-β-actin isolate from a human fetal brain cDNA library and plated onto minimal medium without tryptophan and histidine. Activity of reporter genes was determined by growth on selective media (-L/-W/-H) and by galactosidase activity assay. The presence of the plasmid in the L40 cells was verified by growth on plates lacking tryptophan and leucine. pBTM116-FEZ1/pACT2-FEZ2 pair [39] was used as positive interaction control.