TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| JM103 | Commercial strain used for cloning | 36 |
| DH5αF′ | Commercial strain used for cloning | BRL |
| TOP10F′ | Commercial strain used for cloning | Invitrogen |
| P. syringae pv. phaseolicola | ||
| NPS3121 | Wild type | 29 |
| UIKcat | argK::cat mutant of NPS3121; Tox+ | This study |
| UIFtet | argF::tet mutant of NPS3121; Tox+ | This study |
| AT3 | amtA::aph mutant of NPS3121; Tox− | 9 |
| Plasmids | ||
| pUIKL1 | argK::cat in pU1511 for gene replacement | This study |
| pUIMC1 | argF::tet in pUIGM1 for gene replacement | This study |
| pMW4 | Chloramphenicol cassette | 19 |
| pUIRM504 | Tn5cat delivery plasmid; Tcr Kmr | 14 |
| pUI511 | argK cloned into pUC19 | 21 |
| pUIGM1 | argF cloned into pUC19 | G. Mosqueda, personal communication |
| pUC19 | bla lacZ′ Apr | 36 |