TABLE 1.
Kinetic parameters for CmaA-FLAG and His-CmaA as determined by the PPi exchange assay
| Substrate | CmaA-FLAG
|
His-CmaA
|
||||
|---|---|---|---|---|---|---|
| Km (mM) | kcat (min−1) | kcat/Km (min−1 mM−1) | Km (mM) | kcat (min−1) | kcat/Km (min−1 mM−1) | |
| l-allo-Isoleucine | 0.26 ± 0.02 | 17 ± 0.3 | 65.4 | 1.4 ± 0.2 | 40 ± 6.0 | 28.6 |
| l-Isoleucine | 1.0 ± 0.1 | 2.8 ± 0.1 | 2.8 | 0.12 ± 0.00 | 1.6 ± 0.1 | 13.3 |
| l-Leucine | 7.4 ± 1.1 | 16 ± 3 | 2.2 | NDa | ND | ND |
| l-Valine | 8.2 ± 1.3 | 12 ± 1 | 1.5 | ND | ND | ND |
| d-allo-Isoleucine | 0.84 ± 0.07 | 0.20 ± 0.06 | 0.24 | 4.9 ± 1.1 | 1.0 ± 0.1 | 0.20 |
| d-Isoleucine | 1.1 ± 0.2 | 1.1 ± 0.2 | 1.0 | 4.1 ± 2.1 | 0.80 ± 0.1 | 0.20 |
| dl-Coronamic acid | 1.5 ± 0.3 | 6.7 ± 0.4 | 4.5 | ND | ND | ND |
| dl-Norcoronamic acid | 11 ± 3 | 1.1 ± 0.2 | 0.10 | ND | ND | ND |
a
ND, not determined.