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Kinetic parameters for CmaA-FLAG as determined by the forward PPi assay
| Substrate | Km (mM) | kcat (min−1) | kcat/Km (min−1 mM−1) |
|---|---|---|---|
| l-allo-Isoleucine | 0.04 ± 0.01 | 0.09 ± 0.00 | 2.3 |
| l-Isoleucine | 0.50 ± 0.06 | 0.71 ± 0.03 | 1.4 |
| l-Leucine | 1.5 ± 0.1 | 1.8 ± 0.1 | 1.2 |
| l-Valine | 1.2 ± 0.1 | 1.4 ± 0.1 | 1.2 |
| d-allo-Isoleucine | 5.6 ± 1.0 | 0.51 ± 0.09 | 0.09 |
| d-Isoleucine | 5.3 ± 0.9 | 0.79 ± 0.09 | 0.15 |
| dl-Coronamic acid | 1.0 ± 0.2 | 1.3 ± 0.1 | 1.3 |
| dl-Norcoronamic acid | 4.5 ± 1.0 | 1.8 ± 0.4 | 0.40 |
