TABLE 1.
Bacterial strains and plasmids
| Strain or plasmid | Genotype or description | Reference or source |
|---|---|---|
| Strains | ||
| E. coli | ||
| DH5α | F− φ80dlacZΔM15 Δ(lacZYA-argF)U169 deoR recA1 endA1 hsdR17 (rK− mK+) supE44 thi-1 gyrA69 | 35 |
| RR1 | F−mcrB mrr hsdS20(rB− mB−) ara-14 proA2 lacY1 leuB2 galK2 rpsL20 (Smr) xyl-5 mtl-1 supE44 | 35 |
| BL21(DE3)/pLysS | F−lon hsdSB (rB− mB−) with DE3, a λ prophage carrying T7 RNA polymerase gene and plasmid pLysS containing T7 phage lysozyme gene | 39 |
| B. subtilis | ||
| 168 | trpC2 | 1 |
| IS58 | trpC2 lys-3 | 38 |
| BUG1 | trpC2 lys-3 ΔclpP::spec | 8 |
| BUG2 | trpC2 lys-3 clpX::pMutin4 | 8 |
| BEK38 | trpC2 lys-3 ΔsigB::neo | 19 |
| BEK90 | trpC2 lys-3 ΔclpX::aphA3 | H. Ludwig and E. Krüger, unpublished data |
| BUG8 | trpC2 ΔclpC::tet | QPB418 (31) |
| BHK02 | trpC2 clpP::pX2 | clpP conditional mutant; this study |
| BMM1 | trpC2 clpP::pX2 ΔclpC | This study |
| BUG9 | trpC2 lys-3 amyE::(clpX′ Δ1-bgaB cat) | pDLXB1-1 → IS58; this study |
| BUG10 | trpC2 lys-3 amyE::(clpX′ Δ1-bgaB cat) ΔctsR::aphA3 | pDORF1 → BUG9; this study |
| BUG11 | trpC2 lys-3 amyE::(clpX′ Δ1-bgaB cat) ΔsigB::neo | BEK38 → BUG9; this study |
| BUG12 | trpC2 lys-3 amyE::(clpX′ Δ1-bgaB cat) ΔclpX::aphA3 | BEK90 → BUG9; this study |
| BUG13 | trpC2 lys-3 amyE::(clpX′ Δ2-bgaB cat) | pDLXB1-3 → IS58; this study |
| BUG14 | trpC2 lys-3 amyE::(clpX′ Δ2-bgaB cat) ΔsigB::neo | BEK38 → BUG13; this study |
| Plasmids | ||
| pDL | Insertion vector; Cmr | 45 |
| pDLXB1-1 | pDL containing a 430-bp clpX PCR fragment with both promoters in EcoRI-BamHI sites | This study |
| pDLXB1-3 | pDL containing a 260-bp clpX PCR fragment carrying only a putative σB promoter in EcoRI-BamHI sites | This study |
| pDORF1 | pBluescript SKII carrying 911-bp KpnI-blunt-end and 409-bp blunt-end-BamHI ctsR fragments of flanking regions with a Kmr cassette | 19 |
| pRSETA | E. coli expression vector; Apr | Invitrogen |
| pRSETAclpC | pRSETA containing B. subtilis clpC | This study |
| pRSETAclpE | pRSETA containing B. subtilis clpE | This study |
| pRSETAclpX | pRSETA containing B. subtilis clpX | This study |
| pRSETAclpP | pRSETA containing B. subtilis clpP | This study |
| pX2clpP | pX2 containing a 300-bp B. subtilis clpP fragment | This study |