FIG. 3.

Physical maps of two E. coli-Gordonia shuttle vectors. The 2,332-bp fragment of plasmid pKB1 that is the smallest essential part necessary to confer stable replication in Gordonia was amplified by PCR (open white bar) and cloned into suicide plasmids pBBR1MCS-2 and pBBR1MCS-5 (31), leading to the new vector plasmids pDBMCS-2 and pDBMCS-5, respectively. The genes located on the suicide plasmids are indicated by black arrows. Unique restriction sites present in the MCS of each plasmid are shown.