TABLE 1.
Bacterial strains and plasmids used for this study
| Strain or plasmid | Relevant characteristic(s) | Reference or source (strain no.) |
|---|---|---|
| Strains | ||
| Gordonia species | ||
| G. alkanivorans HKI 0136 | Hexadekane-degrading wild type | 33 (DSM 44369) |
| G. amicalis IEGMT | Dibenzothiophene-desulfurizing strain | 30 (DSM 44461T) |
| G. bronchialis | (DSM 43247T) | |
| G. desulfuricans 213E | Benzothiophene-desulfurizing wild type | 29 (NCIMB 40816) |
| G. nitida LE31T | 3-Ethylpyridine- and 3-methylpyridine-degrading strain | 70 (DSM 44499) |
| G. polyisoprenivorans Kd2 | cis-1,4-Polyisoprene-degrading wild type | 37 (DSM 44302) |
| G. polyisoprenivorans VH2 | cis-1,4-Polyisoprene-degrading wild type | 2 (DSM 44266) |
| G. polyisoprenivorans Y2K | cis-1,4-Polyisoprene-degrading wild type | 2 |
| G. sputi | (DSM 43896T) | |
| G. terrae | Ethyl t-butyl ether-degrading strain | 23 (DSM 43249T) |
| G. westfalica Kb1/Kb2 | cis-1,4-Polyisoprene-degrading wild type | 38 (DSM 44215) |
| G. westfalica Kb1-K12 | pKB1-deficient mutant of G. westfalica Kb1 | This work |
| G. westfalica Kb1-K34 | pKB1-deficient mutant of G. westfalica Kb1 | This work |
| G. westfalica Kb1-K35 | pKB1-deficient mutant of G. westfalica Kb1 | This work |
| G. westfalica Kb1-K36 | pKB1-deficient mutant of G. westfalica Kb1 | This work |
| G. westfalica Kb1-K37 | pKB1-deficient mutant of G. westfalica Kb1 | This work |
| G. westfalica Kb1-K38 | pKB1-deficient mutant of G. westfalica Kb1 | This work |
| G. westfalica Kb1-K39 | pKB1-deficient mutant of G. westfalica Kb1 | This work |
| G. westfalica Kb1-K40 | pKB1-deficient mutant of G. westfalica Kb1 | This work |
| G. westfalica Kb1-K41 | pKB1-deficient mutant of G. westfalica Kb1 | This work |
| G. westfalica Kb1-K42 | pKB1-deficient mutant of G. westfalica Kb1 | This work |
| G. westfalica Kb1-K43 | pKB1-deficient mutant of G. westfalica Kb1 | This work |
| G. westfalica Kb1-K44 | pKB1-deficient mutant of G. westfalica Kb1 | This work |
| G. westfalica Kb1-K45 | pKB1-deficient mutant of G. westfalica Kb1 | This work |
| G. westfalica Kb1-K46 | pKB1-deficient mutant of G. westfalica Kb1 | This work |
| Mycobacterium species | ||
| M. smegmatis mc2155 | Transformation-efficient mutant | 58 |
| Rhodococcus species | ||
| R. opacus PD630 | Wild type; TAG+ | (DSMZ 44193) |
| R. rhodochrous RNMS1 | Wild type | 61 (ATCC 13808T) |
| R. ruber | Wild type; PHA+ TAG+ | 22 |
| Streptomyces species | ||
| S. coelicolor A3(2) | Wild type | 56 (DSMZ 40783) |
| S. lividans TK23 | Wild type | John Innes Institut, Norwick, England |
| E. coli strains | ||
| E. coli XL1-Blue | recA1 endA1 gyrA96 thi1 hsdR17(rK− mK+) supE44 relA1 λ−lac [F′ proAB lacIqlacZΔM15 Tn10 (Tcr)] | 12 |
| E. coli S17-1 | thi1 proA hsdR17(rK− mK+) recA1 tra gene of plasmid RP4 integrated into the chromomsome | 57 |
| E. coli W3110 | Wild type | 20 |
| E. coli RW3110 | zntA | 50 |
| Plasmids | ||
| pASK3 | E. coli vector | IBA-GmbH, Göttingen, Germany |
| pBBR1MCS-1 | CmrlacPOZ′, Mob | 32 |
| pBBR1MCS-2 | KmrlacPOZ′, Mob | 31 |
| pBBR1MCS-3 | TcrlacPOZ′, Mob | 31 |
| pBBR1MCS-4 | Apr, lacPOZ′, Mob | 31 |
| pBBR1MCS-5 | Gmr, lacPOZ′, Mob | 31 |
| pBBRKB1:XhoI7 | Kmr, lacPOZ′, Mob, oriV pKB1 | This work |
| pDBMCS-2 | KmrlacPOZ′, Mob, oriV pKB1 | This work |
| pDBMCS-5 | GmrlacPOZ′, Mob, oriV pKB1 | This work |