TABLE 4.
Transfer of pKb1 oria
| Strain | Method of DNA transfer | Autonomous replicationb |
|---|---|---|
| G. alkanivorans HKI 0136 | Electroporation | − |
| G. desulfuricans 213E | Electroporation | − |
| G. polyisoprenivorans Kd2 | Electroporation | − |
| G. polyisoprenivorans VH2 | Electroporation | + |
| G. polyisoprenivorans Y2K | Electroporation | + |
| G. westfalica Kb1 | Electroporation | − |
| G. westfalica Kb2 | Electroporation | − |
| M. smegmatis mc2155 | Electroporation | + |
| R. opacus PD630 | Electroporation | + |
| R. rhodochrous RNMS1 | Electroporation | − |
| R. ruber | Electroporation | − |
| S. coelicolor A3(2) | Protoplast transformation | − |
| S. lividans TK23 | Protoplast transformation | − |
a
The oriV-containing hybrid plasmid pBBRKB1:XhoI7 (Fig. 2A) was transferred to different members of the genera Gordonia, Rhodococcus, and Mycobacterium by electroporation and of Streptomyces by protoplast transformation. Resistant colonies were screened for the presence of pBBRKB1:XhoI7 by plating on St-I agar plates with 50 mg of kanamycin per liter.
b
+, positive DNA transfer; −, negative DNA transfer.