TABLE 2.
Proteases and hydrolases
| Enzyme and locus | Counta
|
Coveragea | Lb | Mol wt | pI | Description | Fractionc | |
|---|---|---|---|---|---|---|---|---|
| Peptide | Spectrum | |||||||
| Proteases | ||||||||
| BA0261 | 4 | 6 | 16 | 338 | 35,973 | 5.2 | O-Sialoglycoprotein endopeptidase (gcp) | 1 |
| BA0329 | 7 | 19 | 16.1 | 409 | 45,230 | 5.3 | Aminopeptidase AmpS (ampS) | 1, 2 |
| BA1177 | 27 | 66 | 34.6 | 866 | 97,545 | 5.5 | ATP-dependent Clp protease ATP-binding subunit ClpB (clpB) | 1, 2 |
| BA1206 | 36 | 166 | 54.3 | 608 | 70,365 | 5 | Oligoendopeptidase F (pepF-1) | 1, 2, 3 |
| BA1353 | 13 | 33 | 37.2 | 564 | 66,205 | 4.9 | Oligoendopeptidase F, putative | 1 |
| BA2001 | 4 | 5 | 19 | 316 | 34,057 | 5 | Intracellular serine protease | 1 |
| BA2297 | 8 | 22 | 23.7 | 422 | 47,089 | 5 | Peptidase, M20/M25/M40 family | 1 |
| BA3872 | 4 | 9 | 12.7 | 410 | 45,911 | 4.8 | Peptidase T (pepT-1) | 1, 2 |
| BA3922 | 15 | 39 | 41.8 | 428 | 49,163 | 5 | Zinc protease, insulinase family | 1, 2 |
| BA3942 | 12 | 53 | 45.5 | 413 | 47,004 | 5.4 | Zinc protease, insulinase family | 1, 2 |
| BA4368 | 6 | 11 | 16.7 | 372 | 39,610 | 5.2 | Peptidase T (pepT-2) | 1, 2 |
| BA4422 | 10 | 41 | 44.2 | 353 | 38,614 | 5.1 | Proline dipeptidase (pepQ-1) | 1, 2 |
| BA4546 | 25 | 94 | 50.8 | 368 | 40,605 | 5 | Spore germination protease (gpr) | 1, 2 |
| BA4702 | 10 | 36 | 18.8 | 773 | 86,212 | 5.9 | ATP-dependent protease La 1 (lon) | 1 |
| BA4704 | 6 | 19 | 13.8 | 419 | 46,199 | 5 | ATP-dependent Clp protease, ATP-binding subunit ClpX (clpX) | 1 |
| BA4815 | 9 | 39 | 24.1 | 361 | 39,581 | 5.6 | Peptidase, M42 family | 1, 2 |
| BA4861 | 7 | 22 | 29.6 | 365 | 40,660 | 5.2 | Proline dipeptidase (pepQ-2) | 1, 2 |
| BA4933 | 8 | 16 | 23.5 | 371 | 41,819 | 5.1 | Aminopeptidase, putative | 1, 2 |
| BA4947 | 10 | 29 | 45.7 | 357 | 39,155 | 6.5 | Peptidase, M42 family | 1, 2 |
| BA5155 | 3 | 7 | 7.5 | 494 | 53,514 | 4.9 | Cytosol aminopeptidase (pepA) | 1 |
| BA5380 | 10 | 42 | 51.8 | 193 | 21,392 | 5.4 | ATP-dependent Clp protease, proteolytic subunit ClpP (clpP-2) | 1, 2 |
| BA5710 | 8 | 17 | 32.2 | 391 | 41,819 | 8 | Serine protease | 1 |
| Hydrolases | ||||||||
| BA0298 | 12 | 35 | 31.9 | 511 | 55,516 | 5.5 | Phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase (purH) | 1 |
| BA1152 | 2 | 3 | 9.4 | 299 | 33,371 | 7.2 | Fumarylacetoacetate hydrolase family protein | 1 |
| BA1532 | 6 | 11 | 25.9 | 189 | 21,020 | 6.9 | GTP cyclohydrolase I (mtrA) | 1 |
| BA3606 | 2 | 4 | 12.9 | 310 | 34,464 | 8 | Inosine-uridine preferring nucleoside hydrolase family protein | 1, 3 |
| BA3668 | 3d | 4d | 3.5d | 430 | 48,169 | 9.2 | Glycosyl hydrolase, family 18 | 2, 3 |
| BA4188 | 2 | 9 | 12.5 | 257 | 29,755 | 5.3 | Hydrolase, haloacid dehalogenase-like family | 1 |
| BA4271 | 2 | 3 | 8.2 | 268 | 30,097 | 5 | Hydrolase, haloacid dehalogenase-like family | 1 |
| BA5390 | 6 | 16 | 29.2 | 216 | 24,767 | 5.2 | Hydrolase, haloacid dehalogenase-like family | 1 |
a
Data are those obtained from the soluble fraction, unless otherwise stated.
b
L, length (in amino acids).
c
1, soluble fraction; 2, membrane/insoluble fraction; 3, Exo fraction. Multiple listings indicate that the protein was detected in more than one fraction, because of either a wide distribution or incomplete fractionation of samples.
d
From membrane/insoluble fraction.