Fig. 1.

The M3-R increases the activity of ORs. (A to C) Untagged ORs. (D to F) Rho-tagged ORs. Lysates of Hana3A cells that were cotransfected with plasmids encoding the indicated ORs and individual GPCRs from a panel of 22 receptors were subjected to luciferase assays under the following conditions: (A and D) Cells expressing OR-S6 were stimulated with nonanedioic acid (3 μM); (B and E) cells expressing OR-EG were stimulated with vanillic acid (30 μM); and (C and F) cells expressing Olfr62 were stimulated with 2-coumaronone (300 μM). Asterisks indicate those GPCRs that significantly increased the activity of each OR relative to that in cells transfected with a vector control, P < 0.05 after Bonferroni correction. Averages were obtained from triplicate assays, and the experiment was repeated four times.