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. Author manuscript; available in PMC: 2011 Jul 11.
Published in final edited form as: Sci Signal. 2011 Jan 11;4(155):ra1. doi: 10.1126/scisignal.2001230

Fig. 2.

Fig. 2

M3-R mRNA is found in OSNs, and M3-R protein is localized to the cilia of the olfactory epithelium. (A to C) In situ hybridization analysis of the olfactory epithelium demonstrating that M3 mRNA is abundant in olfactory neurons. OMP is a marker for mature OFNs. IL-8RB, which does not hybridize to the OSNs, is used as a control GPCR probe. (D to G) Immunohistochemical analysis with antibodies against the M1-R, M3-R, and M5-R. The M3-R, but not M1-R and M5-R, is abundant at the cilia of the olfactory epithelium. A probe to ACIII was used as a positive control. The experiment was repeated at least three times.