Fig. 5.

(A and B) The M3-R does not increase the cell surface abundance of ORs. HEK 293T cells were cotransfected with plasmids encoding Rho-tagged OR-S6 together with plasmids encoding RTP1S, the M3-R, or both, and the cell surface of OR-S6 was determined by immunostaining (A) or was quantified by flow cytometric analysis (B). The cell surface abundance of ORs increased substantially in cells co-transfected with plasmid encoding RTP1S alone, but not in cells transfected with plasmid encoding the M3-R alone. Cotransfecting cells with plasmids encoding both the M3-R and the RTP1S did not substantially increase the cell surface abundance of either OR when compared to that in cells transfected with plasmid encoding RTP1S alone. Results are from a single experiment; experiments were replicated at least three times (A) or twice (B).