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Potentiation of OR activity by the M3-R is synergistic with that of RTP1S and is independent of tag. (A to D) Dose-response curves of the indicated OR ligands for luciferase assays performed in HEK 293T cells that were transfected with a combination of plasmids encoding RTP1S, Ric-8B, and the M3-R and (A) untagged OR-S6, (B) untagged Olfr62, (C) Rho-tagged OR-S6, or (D) Rho-tagged Olfr62. Error bars indicate the SEM, and assays were performed in triplicate.
