Skip to main content
. Author manuscript; available in PMC: 2011 Jul 11.
Published in final edited form as: Sci Signal. 2011 Jan 11;4(155):ra1. doi: 10.1126/scisignal.2001230

Fig. 9.

Fig. 9

The M3-R and S6 stably interact in HEK 293T cells. Coimmunoprecipitation studies of HA-tagged M3 and FLAG-tagged OR-S6 in HEK 293T cells. (A to D) When cell extracts were used in immunoprecipitation assays with antibody against the HA tag, we could detect FLAG-tagged S6 proteins. (B, lane 3). No coimmunoprecipitation was detected when FLAG-taggedCD28, a negative control, was used (B, lane 4). Likewise, when cell extracts were used in immunoprecipitation assays with antibody against the FLAG tag, we detected HA-tagged M3-R protein (C, lane 5). (E and F) When FLAG-tagged OR-S6 was coexpressed with various HA-tagged GPCRs, OR-S6 could be coimmunoprecipitated with either HA-tagged M2-R or HA-tagged M3-R; however, other GPCRs were not coimmunoprecipitated (F). Data are from single experiments.