Figure 1.

The influence of nucleotides on the oligomeric state of CTP synthase. The molecular weights calculated for the dimer and tetramer from the sequence are 120 and 240 kDa, respectively, which are in good agreement with the molecular weights (105 and 210 kDa) calculated from the sedimentation profiles according to the formula of Martin & Ames (1961 ▶). (a) Sedimentation of CTP synthase and marker enzymes in the presence of nucleotides: diamonds, catalase activity (250 kDa); squares, alcohol dehydrogenase activity (150 kDa); triangles, CTP synthase activity. (b) Sedimentation of CTP synthase and marker enzymes in the absence of nucleotides. Symbols are the same as above.