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Recombinant purification and formation of IgCAM3 crystals. (a) Coomassie Blue stain of recombinant IgCAM3 separated on an SDS–polyacrylamide gel. (b) Western blot for MLCK1. (c) IgCAM3 crystals formed using 28%(w/v) PEG 4000, 0.1 M ammonium acetate pH 4.6, 0.2 M ammonium sulfate.
