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Purification of the Gh-rTDH protein. (a) The crude protein expressed from BL21 (DE3) pLysS strain (lane 1) was passed through Phenyl Sepharose 6 Fast Flow twice (lanes 2 and 3) to obtain a homogenous protein with a molecular mass of ∼22 kDa as shown by SDS–PAGE. (b) Native PAGE of Gh-rTDH, showing a molecular mass of ∼90 kDa; lane 1, crude protein; lane 2, purified protein; lane M, molecular-weight standards (kDa).
