TABLE 2.

Sequence of the primers used to detect deleted regions in the dassie bacillus relative to M. tuberculosis H37Rv^a

Primer	Sequence
	RD5das	RDVirSdas	N-RD25das	RD1das
Forward	AAGAGGCAAAAGCACGTTGT	GATCACCCATTCCTGCATGT	GATCGGCCTTACCCATTTG	AGGAGCGTGAAGAAGACGAC
Reverse	AACGTAGGACGTCTCGGTGA	TAGCGGAACAAGTCCCAGGT	GCTGAGTGCGGAGTATGCTT	CCTATACGTGTTCCCCATCG
Reverse within	CCTACCCGTGGGATTTCAT	GACAGAAGCGGTTCGCATT	ACCAAGGTCATCCCAGACAG	TATCGTCCGGAGCTTCCATA

^aForward and reverse primers amplify if the dassie-specific deletion has occurred. Forward and reverse-within primers amplify if the region is intact, as seen in M. tuberculosis H37Rv reference. All primers are listed in the 5′-to-3′ orientation.