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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2010 Oct 21;31(1):50–57. doi: 10.1161/ATVBAHA.110.210971

Figure 5.

Figure 5

TLR4 deficiency reduces cholesteryl ester accumulation in mouse aortic SMC. A: Intracellular neutral lipid (Oil Red O fluorescence) is abundant in SMC incubated with cyclodextrin-cholesterol (CD-Chol) for 68 h (Blue: Hoescht 33342 nuclear stain). B: TLR4 KO SMC incubated with CD-Chol (10 or 20 µg/ml) show reduced cholesteryl ester content, expressed per µg cellular protein. C: Cholesterol- and LPS-induced ACAT1 gene expression are abolished in TLR4 KO SMC. * P≤ .0002 vs. CON (wild-type SMC without CD-cholesterol or LPS treatment); + P< .0001 vs. SMC treated with CD-cholesterol or LPS.