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. Author manuscript; available in PMC: 2012 Jan 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2010 Oct 21;31(1):50–57. doi: 10.1161/ATVBAHA.110.210971

Figure 6.

Figure 6

Cholesterol enrichment stimulates expression of MCP-1, CD68 and Mrp 8 in ApoE KO SMC: role of TLR4. A: SMC from ApoE KO and TLR DKO mice were incubated without (control; CON) or with cyclodextrin-cholesterol (CD-Chol) for 24 h, and SMA and CD68 expression were determined by immunofluorescence staining. SMC incubated in media alone express abundant SMA (green; top panel), but no CD68 (green; bottom panel). SMA is diminished in SMC incubated with CD-Chol, while some SMC express intracellular CD68 (Blue: Hoescht 33342 nuclear stain). B: MCP-1, CD68, and Mrp8 mRNA levels were determined by real-time RT-PCR analysis, normalized to β-actin mRNA, and expressed relative to CON levels. MCP-1 release by SMC was determined by ELISA. *P≤ . 05 vs. ApoE KO SMC.