Table 2.

Nucleotide sequences of the sense and anti-sense primers as well as the annealing temperatures used in the quantification of known protease genes by real-time RT-PCR

Protease	Sense primer	Anti-sense primer	Annealing (°C)
Tmprss2	TGACTGCTGCTCACTGCTTT	ATGGTTTGCATCTGGGAGAC	52
Tmprss4	AGGGGAGGATGAGGAACACT	ATCTGGACGGATCTCCACTG	52
Tpsg1	GGTCACACTGTCTCCCCACT	ACTTTGGCCTCCTGAAGGTT	52
Ctsd	TGATGGGAGCTGGTTTCAAT	TCATCAGGGCATAGGACACA	50
Elane	GGCTTTGACCCATCACAACT	CGGCACATGTTAGTCACCAC	52
Ctrl	CCCATTGCCTCAGCAACTAT	CCAGCCTGTGACATAGCAGA	52
Mmp 1a	CCTTCCTTTGCTGTTGCTTC	CACCTGGGCTTCTTCATAGC	52
Mmp 1b	GTGCTCTCCTTCCACAGAGG	ATGGGAGAGTCCAAGGGAGT	52
Mmp 2	GAAACCGTGGATGATGCTTT	CCATCAGCGTTCCCATACTT	50
Mmp 8	AACGGTCTTCAGGCTGCTTA	GGGAACATGCTTGGTATGCT	52
Mmp 9	CGTCGTGATCCCCACTTACT	AACACACAGGGTTTGCCTTC	52
Gzma	TGATGTGAAACCAGGAACCA	ATGCCTCGCAAAATACCATC	50
Gzmb	GACCCAGCAAGTCATCCCTA	CACACTCCCGATCCTTCTGT	54
Gzmc	CCAGGGGATGAGTGCTATGT	ATCCATCAGTTTGCCCGTAG	52
Gzmg	CATTCCCCATCCAGCTTTTA	GATCTGCGTGGTCTTGGAAT	50
Gzmk	CCGTGGTTTTAGGAGCACAT	CAGGGTATCAGAGGCGGTTA	52
Actb	GTCCCTCACCCTCCCAAAAG	GCTGCCTCAACACCTCAACCC	55
Gapdh	GGTGAAGGTCGGTGTGAACG	CTCGCTCCTGGAAGATGGTG	55