Table 3.
Nucleotide sequences of the sense and anti-sense primers as well as the annealing temperatures used in the quantification of the viral HA1 and HA7 transcripts by real-time RT-PCR
Target | Sense primer | Anti-sense primer | Annealing (°C) |
---|---|---|---|
HA1 | CAGATGCAGACACAATATGT | TAGTGGGGCTATTCCTTTTA | 48 |
HA7 | TCTGCCATTCCAAAACATCA | GCAGTTCCTTCTCCTTGTGC | 48 |