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. 2011 Feb 7;6(2):e14661. doi: 10.1371/journal.pone.0014661

Figure 2. Overexpression of PKCδ sensitizes NB cells to BSO-induced apoptosis.

Figure 2

A, After transfections and treatments (performed as in Fig. 1), apoptosis was analyzed by fluorescence microscopy using Annexin-V/PI assay (see Materials and Methods). Histograms summarize quantitative data of means ± SD of five independent experiments. * p<0.01 vs. EV transfected cells; ° p<0.01 vs. PKCδ transfected cells; § p<0.01 vs. EV transfected cells +BSO; # p<0.01 vs PKCδ transfected cells +BSO. B, Caspase-3 activity was analyzed by Caspase-3 Cellular Activity Assay Kit (see Materials and Methods) in SH-SY-5Y (left panel) and SK-N-BE-2C cells (right panel). The percentage of living cells was determined by MTT analyses. Histograms summarize quantitative data of means ± SD of three independent experiments. * p<0.05 vs. EV transfected cells; ** p<0.01 vs. EV transfected cells; ° p<0.05 vs. PKCδ transfected cells; °° p<0.01 vs. PKCδ transfected cells.