Figure 2. Optimizing MOI.

To optimize the MOI, Sf21 cells were infected with all 4 constructs at a MOI of 1 (white bars), 2 (light gray bars), 3 (dark gray bars) and 4 (black bars). NA activity in the media was measured at 0 h, 24 h, 48 h and 72 h. Increasing the MOI did not have any significant impact on the amount of secreted NA activity. This experiment was performed once in duplicate.