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. 2011 Feb 7;6(2):e16284. doi: 10.1371/journal.pone.0016284

Figure 3. Characterization of secreted NA in media.

Figure 3

(A) The different NA-containing media were incubated for 3 days on ice, RT, or 37°C. The secreted soluble construct 2–4 were stable at RT but not at 37°C whereas the membrane bound full length Hokkaido N1 was stable at all temperatures. (B) Centrifugation of construct 2–4 containing media for 1 h at 40,000 g did not affect the amount of detected NA activity. (C) Incubation of construct 2–4 containing media at pH 2.8 abolished most of the NA activity of construct 2–4 within 1 min. This experiment was performed once in duplicate.