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. 2011 Feb 7;6(2):e16284. doi: 10.1371/journal.pone.0016284

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Schmidt et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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To optimize the expression system Sf21 cells were compared to Hi5 cells. (A) There was no significant difference in the amount of secreted NA activity observed for construct 3 or 4 in both cell lines. (B) Cell viability seemed to drop faster for Hi5 compared to Sf21 cells without affecting the amount of secreted NA activity. (C, D) Anti-FLAG western blot of construct 3- or 4-containing media showed a strong signal for both cell lines. Activity and viability data are duplicates of three independent infections. For the respective western blots the supernatants of all three infections were pooled.