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. 2011 Feb 7;6(2):e16284. doi: 10.1371/journal.pone.0016284

Figure 5. Large scale expression and purification exemplified by construct 4.

Figure 5

To obtain sufficient amounts of NA, larger (5l) Sf21 cultures were infected and the respective NA was purified from the media. (A) UV protein imaging and (B) western blot of the 40,000 g media samples following the NA expression over 96 h. (C, D) Peptide eluted fractions from the anti-FLAG affinity matrix stained by Coomassie (C) or anti-FLAG western blot (D). (E) Gel filtration chromatogram of the FLAG-purified construct 4 recorded at 280 nm wavelength. (F) Purified construct 4 stained by Coomassie (left panel) or by anti-FLAG western blot (right panel).