Table 1. Biochemical properties of different NAs.
| Hokkaido virus | Construct 2 | Construct 4 | ||
| N-terminal sequencing | - | AEFDYKDDD… | AEFDYKDDD… | |
| specific activity (pmol/sec/µg) | - | 387±26.7 | 377±12.7 | |
| turnover number (molecules/sec/head) | - | 79.4±5.5 | 77.2±2.6 | |
| K m value for MUNANA (µM) | 24.1±1.1 | 54.1±4.4 | 34.7±1.9 | |
| IC50 | oseltamivir (nM) | 3.72±0.45 | 12.5±1.9 | 8.67±1.33 |
| zanamivir (nM) | 1.37±0.20 | 11.6±1.8 | 4.93±0.73 | |
| peramivir (nM) | 0.37±0.03 | 1.79±0.21 | 0.82±0.1 | |
| K i | oseltamivir (nM) | 3.78±0.20 | 5.37±0.96 | 3.70±0.05 |
| zanamivir (nM) | 1.28±0.07 | 3.85±0.75 | 3.19±0.22 | |
| peramivir (nM) | 0.50±0.03 | 1.42±0.04 | 0.51±0.13 | |
The table shows the determined biochemical properties of the original Hokkaido virus NA and the artificial NAs derived from this virus. In addition, the IC50 and K i values for the common NA-inhibitors oseltamivir, zanamivir, and peramivir are shown for all 3 NAs.