Table 2. Primers used to amplify and fuse artificial stalk and NA head.

Name	direction	Sequence
Construct 1	forward	5-GCATCGGAATTCCTGAAGCAGATCGAGG-3
Construct 2/4	forward	5- GCATCGGAATTCGACTACAAGGACGACG-3
Construct 3	forward	5- GCATCGGAATTCGGTTCCATCATCAACG-3
Hokkaido-Stalk	forward	5-CAACACCAATGTTATTGCTGGAAAGGAC-3
Hokkaido-Stalk	reverse	5-CACCAATGTTATTGCTGGAAAGGACAAAACTTC-3
Hokkaido-Head	reverse	5-GCTCTAGATTACTACTTGTCAATGGTGAATGGCAAC-3

All primers are shown in 5′ to 3′ direction. The underlined nucleotides represent the restriction sites (EcoRI, XbaI) used for subsequent cloning into pFastBac-MSP.