Figure 4. Characterization of 5IA85380 endogenous GABA release.

(A) Effect of DHβE, MLA, TTX and SKF 89976A on endogenous GABA overflow evoked by 5IA85380 from rat hippocampal synaptosomes. Synaptosomes were depolarised with 5IA85380 for 90 s at t = 38 min of superfusion. When appropriate, antagonists were introduced 8 min before depolarization. Data are mean ± SEM of 3–6 experiments run in triplicate. ***p<0.001 versus 5IA85380 evoked GABA overflow. One way ANOVA followed by Dunnett post hoc test. (B) Effect of Ca²⁺ free, Cd²⁺ and thapsigargin, dantrolene, and xestospongin C on endogenous GABA overflow evoked by 5IA85380 from rat hippocampal synaptosomes. When appropriate, antagonists were introduced 8 min before 5IA85380. Data are mean ± SEM of 3–6 experiments run in triplicate. ***p<0.001 versus 5IA85380 evoked GABA overflow. One way ANOVA followed by Dunnett post hoc test.