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. Author manuscript; available in PMC: 2011 Feb 7.
Published in final edited form as: Chem Biol. 2009 Oct 30;16(10):1045–1052. doi: 10.1016/j.chembiol.2009.09.012

Figure 1. Pristimerin inhibits MGL.

Figure 1

(A) Concentration-dependent inhibition of purified MGL (squares, n=3) and HeLa MGL (circles, n=4) by pristimerin.

(B) Rapid dilution assays of HeLa-MGL in the presence of vehicle (squares, DMSO, final concentration 2%), pristimerin (triangles), or MAFP (circles). We measured the amount of reaction product (pmol/μg protein) generated over a 60-min period following a 20-min preincubation with inhibitor or vehicle (n=3).

(C) Time-course of MGL inhibition by pristimerin (300 nM, n=3).

(D) Michaelis-Menten analysis of the MGL reaction (measured as pmol/min/μg protein) in the presence of vehicle (squares, DMSO, 1%) (n=4) or pristimerin (100 nM, 300 nM, or 500 nM; n=3-4).

Enzyme activity is reported as percent of vehicle controls (DMSO, 1%). Results are expressed as mean ± SEM, with each assay performed in duplicate.