. Author manuscript; available in PMC: 2012 Feb 1.

Published in final edited form as: J Microbiol Methods. 2010 Dec 1;84(2):174–182. doi: 10.1016/j.mimet.2010.11.015

Table 2.

Ingredient list of R slant and broth.

Slant Ingredient	Quantity	Broth Ingredient	Quantity
Before autoclaving pH 6.7
Brucella broth	28 g/L	Brucella broth	28g/L
Agar	7 g/L	HEPES	10 mM
HEPES	10 mM	CuSO₄	0.2 μM
CuSO₄	0.2 μM	MnSO₄	0.2 μM
MnSO₄	0.2 μM	ZnSO₄	3 μM
ZnSO₄	3 μM

After autoclaving
FeSO₄	50 μM	FeSO₄	50 μM
MgCl₂	250 μM	MgCl₂	250 μM
Sodium pyruvate	250 mg/L	Sodium pyruvate	250 mg/L
		Human blood serum	2%
		Human blood lysate	2%