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. Author manuscript; available in PMC: 2012 Feb 7.
Published in final edited form as: Mol Pharm. 2010 Dec 17;8(1):44–49. doi: 10.1021/mp100230y

Figure 2.

Figure 2

Construction and verification of HSV recombinant C150. A. Schematic diagrams depicting genomic structure of wildtype HSV-1 (F), and the γ134.5 region within recombinant viruses C101, C130, C140 and C150. The diagram also indicates that the inverted repeat region (b’a’) contains the same deletion, restriction sites and/or inserted genes as in the terminal a sequence region. Pm; PmeI site, N; NcoI site. B: Southern blot of viral DNA isolated from ten C150 candidates (1–10) digested with NcoI. HSV-1”F”, C101, C130 and C140 were used as control viral DNAs. Plasmid pRB4794, which contains the 1.8 kb NcoI fragment within the γ134.5 gene, was used as probe. Predicted hybridization band size for C150 candidate viruses is about 1.2 kb. A faint band around 1 kb will also be seen in positive candidates if sufficient quantities of vDNA are loaded (clones 7, 10).