Fig. 6.

Human beta cell apoptosis was not increased in grafts exposed to hyperglycaemia. a Sections from a mouse pancreas 6 h after intraperitoneal streptozotocin injection and (b, c) from human islet grafts, stained for insulin (red) and TUNEL (green) allowed quantification of apoptosis. Confocal images are representative examples of TUNEL staining of positive control. Scale bars 10 μm. d Quantification of TUNEL staining did not reveal a relationship between beta cell apoptosis and circulating blood glucose or (e) plasma human insulin, p=NS for both. Two mice (e) did not have plasma insulin measured, and three points are not visible due to grafts with identical TUNEL and insulin values